Cell culture media prep
Reconstitute powdered media and dilute concentrated stocks where pyrogen contamination at sub-EU/mL levels would activate TLR4 and skew assay readouts.
RW-EFC-100
Endotoxin-Free Cell Culture Water
<0.005 EU/mL endotoxin for sensitive in-vitro work. Sterile-filtered through 0.1 µm and qualified for media preparation, cell wash buffers, and pyrogen-sensitive workflows.
$24.99
$249.90 / L · CofA included
Pack size
Endotoxin < 0.005 EU/mL
CofA per lot
Mycoplasma-tested
0.1 µm sterile-filtered
Specifications
Endotoxin< 0.005 EU/mL (LAL kinetic chromogenic)
Filtration0.1 µm sterile-filtered
MycoplasmaPCR-tested mycoplasma-negative per lot
QC qualificationTissue-culture qualified; bacterial bioburden zero
ContainerTissue-culture-grade HDPE bottle, tamper-evident closure
Shelf life24 months sealed
Storage15 – 25 °C, protect from light
Lot trackingLot number printed on bottle; CofA accessible at /lab-results/
Applications
Where this SKU is used in research workflows.
Primary cell washing
Wash and resuspend primary cells, including macrophages, dendritic cells, and PBMCs, where ambient endotoxin would compromise viability or innate-immune readouts.
Hybridoma & organoid work
Suitable for hybridoma cultivation, organoid buffers, and 3D culture workflows where small endotoxin spikes destabilize differentiation programs.
In-vitro cytokine assays
Cytokine ELISAs, multiplex panels, and stimulation assays demand pyrogen-free reagents to keep baseline IL-6, TNF-α, and IL-1β below detection limits.
Research use only. This product is not for human or veterinary consumption, clinical use, diagnostic procedures, or therapeutic purposes. By purchasing, you affirm you are a qualified researcher or laboratory professional aged 21 or older.
Common questions
About this product.
What endotoxin level should I look for in cell culture water?
For routine cell culture, <0.05 EU/mL is acceptable. For pyrogen-sensitive work — primary monocytes, macrophages, organoids, cytokine assays — <0.005 EU/mL is the recognized threshold. This SKU meets the stricter spec on every lot.
Is it mycoplasma-tested?
Yes. Each lot is screened by PCR for the most common contaminating mycoplasma species and must be negative before release. The CofA documents the assay used and the result.
Why pick this over Type I or sterile water?
Type I water and standard sterile water are not specified for endotoxin or mycoplasma. They may meet the spec on a given lot, but it is not part of the QC release criteria. This SKU adds endotoxin (<0.005 EU/mL), mycoplasma negativity, and tissue-culture qualification as release-controlled specs.
How do I download the Certificate of Analysis?
Visit our Lab Results portal and enter the lot number printed on the bottle. You'll receive the full CofA — including endotoxin LAL results, mycoplasma PCR, and bioburden release — as a downloadable PDF.