PCR / qPCR / RT-PCR
Master mix preparation and template dilution for PCR, qPCR, and RT-PCR where trace nuclease activity would degrade templates, primers, or transcripts.
RW-NF-50A
Nuclease-Free Water
PCR-certified, nuclease-free aliquots for molecular biology. UV-treated, 0.1 µm filtered, autoclaved in dispensed container. Functionally tested for PCR and RT-PCR per lot.
$39.99
$0.80 / aliquot · CofA included
Pack size
PCR-certified
CofA per lot
DNase/RNase-free
Endotoxin < 0.005 EU/mL
Specifications
Nuclease activityDNase, RNase, and protease-free
Endotoxin< 0.005 EU/mL (LAL kinetic chromogenic)
Filtration0.1 µm sterile filtered
TreatmentDEPC-treatment-free formulation; UV-treated and autoclaved
QC releasePCR / RT-PCR functionally tested per lot
ContainerPre-aliquoted polypropylene tubes (1.5 / 10 mL); HDPE bottle (500 mL)
Shelf life24 months sealed
Lot trackingLot number printed on container; CofA accessible at /lab-results/
Applications
Where this SKU is used in research workflows.
NGS library prep
End-to-end use across NGS library construction — fragmentation, end-repair, ligation, bead washes — without introducing nuclease or endotoxin contamination.
In-vitro transcription
Diluent for IVT reactions where RNase contamination at any level destroys transcript yield. Pre-aliquoted format eliminates cross-contamination from shared bottles.
Cloning & primer dilution
Resuspend and dilute primers, oligos, and plasmid stocks for cloning workflows where DNA integrity drives downstream transformation efficiency.
Research use only. This product is not for human or veterinary consumption, clinical use, diagnostic procedures, or therapeutic purposes. By purchasing, you affirm you are a qualified researcher or laboratory professional aged 21 or older.
Common questions
About this product.
Is this DEPC-treated?
No. We use a DEPC-treatment-free process: UV irradiation, 0.1 µm filtration, and terminal autoclaving in the dispensed container. Each lot is functionally tested for PCR and RT-PCR activity. DEPC treatment is unnecessary when the upstream process is validated nuclease-free.
What does "PCR-certified per lot" mean?
Each manufacturing lot is run through a functional PCR and RT-PCR assay using known-yield templates. Lots that don't return target Ct values within spec are rejected. The CofA documents both the molecular biology specs and the functional release results.
Should I aliquot the 500 mL bottle myself?
If your workflow can tolerate the introduction of pipette-tip-borne nucleases between draws, the 500 mL bottle is fine. For RNA-sensitive work — IVT, RNA-seq library prep, single-cell — choose the pre-aliquoted 1.5 mL or 10 mL format to eliminate cross-contamination risk entirely.
How do I download the Certificate of Analysis?
Visit our Lab Results portal and enter the lot number printed on the container. You'll receive the full CofA — including PCR/RT-PCR release data, endotoxin, and nuclease activity assays — as a downloadable PDF.